B. Sc. A.H. (Hons.)

Course Code: BMB 1103   

Course Title: Chemistry of Biomolecules

Credit: 3 (Three)

Contact Hours: 3 hours per week

Level: 1 Semester: 1

 

Biophysical properties of water.  Acids, bases and ampholytes.  pH and buffer. Colloidal properties of matter. Diffusion and osmosis.

Carbohydrates: Occurrence, Functions and classifications. Ring size determination. Homo and heteropolysaccharides. Enzymatic degradation of starch and cellulose.

Proteins: Definition and Classification of amino acids. Naturally occurring peptides. Reactions related to sequence determination. Organization levels. Denaturation.

Lipids: Classification and functions. Distribution of fatty acids in nature. Fats, Oils and waxes. Characterization of fat. Essential fatty acids and prostaglandins and glycolipids as membrane constituents.

Nucleic acids: Occurrence, classification, composition, physicochemical properties and biological functions. DNA as a genetic material. Central dogma of genetic information.

Enzymes: Classification and properties. Mode of action. Elements of kinetics. Factors affecting enzyme activity. Enzyme inhibition. Allosteric enzyme. Enzymes in dairy industry.

Animal Hormones: Chemical nature. Classification and biochemical function.

 

Books Recommended:

  1. Conn, E.C. AND Stump, P.K. 1987. Outlines of Biochemistry. 5th J. Wiley and Sons, New York.
  2. Eliot, W.H. and Elliot, D.C. 1997. Biochemistry and Molecular Biology.
  3. A.I. 1976 Textbook of Biochemistry. 2nd ed. Worth Publishers, New York.
  4. A.I. 1980 Principles of Biochemistry. Worth Publishers, New York.
  5. Martin, D.W., Mayes, P.A. and Rodwell, V.W. 1981. Herper`s Review of Biochemistry 18 th Lange Medical Pub. California.
  6. Stryer, L. 1986. Biochemistry. S.K.Jain publishers, Delhi, India.
  7. Watson, D. 1987. Molecular Biology of Gene. Bengamin, Inc.

 

 

Course Code: BMB 1104   

Course Title: Chemistry of Biomolecules

Credit: 2 (Two)

Contact Hours: 2 hours per week

Level: 1 Semester: 1

 

Preparation of solutions and their standardization.

Identification of Carbohydrates.

Protein colour tests.

Starch preparation and action of salivary amylase.

Fehling`s method of glucose estimation.

Solubility tests of fats.

Salting out of albumins and globulins.

Biuret method of protein estimation.

 

Books Recommended:

  1. David Glick, 1995. Methods of Biochemical Analysis. Interscience Publishers, Inc. N.Y.
  2. Litwack, G. 1960. Experimental Biochemistry for students. Lypee brothers, N.Y.
  3. Malhotra, V.K. 1968. Practical Biochemistry for students. Lypee brothers, N.Y.
  4. Plummer, D.T. 1995. An Introduction to Practical Biochemistry. Tara Mcgraw-Hill. Delhi.
  5. Segal, I.H. 1968. Biochemical Calculations. John Wiley and Sons. New York.

 

Course Code: BMB 1205   

Course Title: Metabolism of Biomolecules

Credit: 2 (Two)

Contact Hours: 2 hours per week

Level: 1 Semester: 2

 

Bioenergetics: Free energy, Entropy and Enthalpy. Exergonic and endergonic reactions. ADP-ATP cycle.

Digestion and absorption of biomolecules: In ruminant and non-ruminant.

An overview of metabolism: Stages in the breakdown of biomolecules.

Carbohydrate metabolism: Glycolytic pathway and fermentation. Citric acid cycle and its amphibolic nature. Anaplerotic reactions. Metabolic regulation of glycolysis and citric acid cycle. Phosphorylation and electron transport chain. Gluconeogenesis. Pentose phosphate pathway and its relation to milk synthesis in mammary gland, Lactose biosynthesis.

Protein metabolism: Sources and utilization of amino acid. Catabolism of amino acids. Detoxification of ammonia in liver and brain. Inborn error of amino acid metabolism.

Lipid Metabolism: Beta-oxidation of fatty acids. Alpha-oxidation in animals. Catabolism of add carbon fatty acids in animals. Ketone bodies formation and their utilization. Biosynthesis of fatty acids.

Nucleic acid metabolism: Protein biosynthesis. Concept of recombinant DNA technology.

Interrelationship of carbohydrate, protein, and lipid metabolism.

 

Books recommended:

  1. Lehninger Principles of Biochemistry by David L. Nelson, 2005 4th W.H. freeman and company. New York.
  2. Principles of Biochemistry by Albert L. Lehninger, 1982. 2nd Worth publishers, Inc. USA. Printed in India
  3. Textbook of Biochemistry by Thomas M. Devlin,2002. 5th John Wiley and sons, Inc. Printed in USA
  4. Biochemistry of Donald Voet, 1995. 2nd edition, John Wiley and sons , New York
  5. Outlines of Biochemistry by Eric E Conn, 1987. John Wiley and sons , New York
  6. Biochemistry by Albert L Lehninger, 1982. 2nd edition Kalyani Publisher. Ludhiana, New Delhi.
  7. Harper`s Biochemistry by Robert K Murry, 2002. 25th McGraw Hill. Printed in Singapore.
  8. Biochemistry by Lubert Stryer, 1995. 3th W. H. freeman and company. New York.
  9. Biochemistry by Mary K Campbell, 1993. 3rd J/B Woolsey Associates. Printed in USA.