DVM

Course Code and Title

BMB 1101, Biophysics and Chemistry of Biomolecules

Pre-requisite course

--

Course Credit

Theory, credit 2; 2 hours per week

Level and Semester

Level – 1 Semester – 1

Course objectives

The students will be equipped with –

• understanding the physiological process of living systems
• knowledge on sources and classification of biomolecules
• concept to explain properties and biochemical functions of biomolecules
• knowledge on constructing structure of biomolecules

Course ILOs [3-5 ILOs for each course ; students perspective]

At the end of the course the student should be able to :

1. Describe various physiological processes of living systems
2. Explain biological functions, physical and chemical properties of biomolecules
3. Compare and contrast among various biomolecules
4. Determine the structure of unknown biomolecules
5. Construct oligomeric -/polymeric forms of various biomolecules

Course Content

[Mention specific topics. Select topics to achieve the stated course objectives and course ILOs]

Course ILOs to be addressed

Number of Lectures / practical sessions for each topics

Law of mass action. Water, acids, bases and electrolytes, pH and buffers. Surface tension. Viscosity. Adsorption. Colloidal state and membrane phenomenon. Diffusion and Osmosis. Spectrophotometry, Electrophoresis, Isoelectric focusing chromatography.

1

10

Carbohydrates: Classification, structural features and biological importance of carbohydrates and their derivatives, bacterial cell wall polysaccharides.

1

2

3

4

5

7

Proteins:  Classification and physiochemical properties of amino acids and proteins, concept of protein structures, protein precipitation, naturally occurring peptides

1

2

3

4

5

5

Lipids: Classification and biological functions; chemistry of fatty acids, fats, phospholipids, sphingolipids, glycolipids, lipoproteins and sterols; lipids as membrane constituent, characterization of fat.

1

2

3

5

Nucleic acids: Classification, structural features and physicochemical properties.

1

2

3

3

Hormones: Classification and biochemical functions.

1

2

3

2

Teaching methods

Lecture, question and answer, audio – visual presentation and assignments

Assessment methods

Multiple choice questions, true / false statements, fill in the blanks, short questions, broad questions, presentation

Books recommended

1. Lehninger Principles of Biochemistry. David L. Nelson and Michael M. Cox. 2017. 7^th W. H. freeman and company. New York.
2. Textbook of Biochemistry by Thomas M. Devlin, 2002. 5^th John Wiley and Sons, Inc. Printed in USA
3. Biochemistry by Donald Voet, 1995. 2^nd edition, John Wiley and Sons, New York
4. Outlines of Biochemistry by Eric E. Conn and Paul K. Stumpf, 1987. 5^th John Wiley and Sons, New York
5. Biochemistry by Albert L Lehninger, 1982. 2^nd edition Kalyani Publishers. Ludhiana, New Delhi.
6. Harper’s Biochemistry by Robert K Murray, 2002. 25^th McGraw Hill. Printed in Singapore.
7. Biochemistry by LubertStryer, 1995. 4^th W. H. freeman and company. New York.
8. Biochemistry by Mary K Campbell, 1999. 3^rd J/B Woolsey Associates. Printed in USA
9. TextBook of Biochemistry by Edward S. West, Wilber R. Todd, Haward S. Mason and John T. Van Bruggan. 4^th Edition, 1966. The MaCmillan Company. Collier-MaCmillan Ltd. London.

Course Code and Title

BMB 1102, Biophysics and Chemistry of Biomolecules

Pre-requisite course

---

Course Credit

Practical, Credit 1; 2 hours per week

Level and Semester

Level – 1 Semester - 1

Course objectives

This course will develop the practical skill on various biochemical methods so that students can use their acquired lab skill for various biochemical researches

Course ILOs [3-5 ILOs for each course ; students perspective]

At the end of the course the student should be able to :

1. Prepare various types of solutions
2. Identify unknown biomolecules in samples
3. Determine the pH of a solution
4. Determine reducing sugar, vitamin and amino acid
5. Detect the existence of sugar and protein in various animal specimens.

Course Content

[Mention specific topics. Select topics to achieve the stated course objectives and course ILOs]

Course ILOs to be addressed

Number of Lectures / practical sessions for each topics

Preparation of solutions and their standardization

1

2

Preparation of buffer solutions and determination of pH

1

3

2

Qualitative analysis of carbohydrates and proteins

2

4

Separation and identification of sugars and amino acids by TLC/paper chromatography

2

2

Quantification of reducing sugars

4

2

Detection of sugars and proteins in animal blood and urine

5

1

Estimation of water-soluble vitamin (ascorbic acid)

4

3

Teaching methods

Lecture, question and answer, demonstration, group discussion, practice

Assessment methods

Multiple choice questions, true / false statements, fill in the blanks, short questions, mathematical problems, presentation

Books recommended

1. David T. Plummer.1995. An introduction to practical Biochemistry. Tata McGraw-Hill Publishing Company Limited, New Delhi.
2. M. Strong 1965. Biochemistry Laboratory Manual. WM.C. Brown Company Publishers, USA.
3. Sterling Chaykin 1970. Biochemistry Laboratory Technioques. Wiley Eastern Private Limited, New Delhi.
4. Irwin H. Segel 1968.Biochemical Calculations. How to Solve Mathematical Problem in General Biochemistry. John Wiley and Sons, Inc. New York.
5. Gerald Litwack 1960.Experimental Biochemistry. A Laboratory Manual. John Liley and Sons. Inc, New York.
6. Official Methods of Analysis. Association of Official Analytical Chemists (AOAC), Washington C., 1990.
7. Varley, H; Gowelock, A.H. and Bell, M. 1980. Practical Clinical Biochemistry. Vol. 1. William Heinemann Medical Books Ltd. London, U.K.

Course Code and Title

BMB 1203, Metabolism of Biomolecules

Pre-requisite courses

BMB 1101, Biophysics and chemistry of Biomolecules

Course Credit

Theory, credit 2; 2 hours per week

Level and Semester

Level – 1 Semester - 2

Course objectives

The students will be equipped with –

• Knowledge on enzyme, enzymatic processes, enzyme and energy kinetics and various metabolic processes of biomolecules
• Concept of interrelation among various metabolic processes
• Disease diagnosis due to nutrient deficiency and metabolic disorders

Course ILOs [3-5 ILOs for each course ; students perspective]

At the end of the course the student should be able to :

1. Describe various metabolic processes (cycles / pathways), energy kinetics, enzymatic processes and enzyme kinetics
2. Construct relationship among various related processes involved in metabolism of different molecules

Course Content

[Mention specific topics. Select topics to achieve the stated course objectives and course ILOs]

Course ILOs to be addressed

Number of Lectures / practical sessions for each topics

Bioenergetics: Free energy, entropy and enthalpy. Exergonic and endergonic reactions. ADP-ATP cycle.

1

3

Enzymes: Classification, co-factors, mode of action, kinetics, inhibition and clinical importance of enzymes

1

2

5

Overview of metabolism: concept of digestion, absorption and metabolism

1

2

2

Carbohydrate metabolism: Glycolysis, fermentation, shuttle system, TCA cycle, electron transport chain, pentose phosphate pathway, gluconeogenesis.

1

2

8

Protein metabolism: Basic processes of amino acid catabolism, ammonia toxicity and its detoxification

1

2

5

Lipid metabolism: Biological oxidation of lipid, propionate catabolism in animals, ketosis, biosynthesis of fatty acids.

1

2

6

Nucleic acid metabolism: Replication, transcription and translation, concept of recombinant DNA technology and molecular cloning

1

2

3

Teaching methods

Lecture, question and answer, audio – visual presentation and assignments

Assessment methods

Multiple choice questions, true / false statements, fill in the blanks, short questions, broad questions, presentation

Books recommended

1. Lehninger Principles of Biochemistry by David L. Nelson, 2008. 5^th W. H. freeman and company. New York.
2. Textbook of Biochemistry by Thomas M. Devlin, 2002. 5^th John Wiley and Sons, Inc. Printed in USA.
3. Biochemistry by Donald Voet, 1995. 2^nd edition, John Wiley and Sons, New York.
4. Outlines of Biochemistry by Eric E. Conn and Paul K. Stumpf, 1987. 5^th John Wiley and Sons, New York.
5. Biochemistry by Albert L Lehninger, 1982. 2^nd edition Kalyani Publishers. Ludhiana, New Delhi.
6. Harper’s Biochemistry by Robert K Murray, 2002. 25^th McGraw Hill. Printed in Singapore.
7. Biochemistry by LubertStryer, 1995. 4^th W. H. freeman and company. New York.
8. Biochemistry by Mary K Campbell, 1999. 3^rd J/B Woolsey Associates. Printed in USA.
9. TextBook of Biochemistry by Edward S. West, Wilber R. Todd, Haward S. Mason and John T. Van Bruggan. 4^th Edition, 1966. The MaCmillan Company. Collier-MaCmillan Ltd. London.

Course Code and Title

BMB 1204, Metabolism of Biomolecules

Pre-requisite course

BMB 1102, Biophysics and Chemistry of Biomolecules

Course Credit

Practical, Credit 1; 2 hours per week

Level and Semester

Level – 1 Semester - 2

Course objectives

This course will develop the practical skill on various biochemical methods so that students can use their acquired lab skill for various biochemical researches

Course ILOs [3-5 ILOs for each course ; students perspective]

At the end of the course the student should be able to :

1. Determine IpH, protein content (soluble and total), saponification value, iodine value, acid value, serum cholesterol, phosphorus and DNA
2. Isolate DNA, serum albumin and globulin
3. Analyze enzymatic activity (salivary amylase, AST and ALT)
4. Prepare media for culturing of bacteria

Course Content

[Mention specific topics. Select topics to achieve the stated course objectives and course ILOs]

Course ILOs to be addressed

Number of Lectures / practical sessions for each topics

Separation of albumins and globulins

2

1

Determination of isoelectric pH of protein

1

2

Quantification of  proteins: Kjeldahl and biuret method

1

3

Qualitative evaluation of animal fats: saponification number, acid value and iodine value

1

3

Activity of salivary amylase

3

2

Extraction and quantification of DNA

1

2

1

Preparation of LB media and culture of E. coli

4

1

Estimation of cholesterol

1

1

Estimation of serum phosphorus

1

1

Assay of AST and ALT

3

1

Teaching methods

Lecture, question and answer, demonstration, group discussion, practice

Assessment methods

Multiple choice questions, true / false statements, fill in the blanks, short questions, mathematical problems, presentation

Books recommended

1. David T. Plummer 1995.An introduction to practical Biochemistry. Tata McGraw-Hill Publishing Company Limited, New Delhi.
2. Irwin H.Segel 1968. Biochemical Calculations. How to Solve Mathematical Problem in General Biochemistry. John Wiley and Sons, Inc. New York.
3. M. Strong 1965.Biochemistry Laboratory Manual. WM.C. Brown Company Publishers, USA.
4. Sterling Chaykin 1970. Biochemistry Laboratory Technioques. Wiley Eastern Private Limited, New Delhi.
5. Gerald Litwack 1960. Experimental Biochemistry. A Laboratory Manual. John Liley and Sons. Inc, New York.
6. Official Methods of Analysis. Association of Official Analytical Chemists (AOAC), Washington D.C., 1990.
7. Verley, H.; Gowelock, A.H. and Bell, M. 1980. Practical Clinical Biochemistry. Vol. 1. William Heinemann Medical Books Ltd. London, U.K.