B. Sc. Ag. (Hons.)

Course Code: BMB 1201

Course Title: Biochemistry and Molecular Biology

Credit: 2 (Two)

Contact Hours: 2 Hours per week

Level: 1 Semester: 2

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale:

The students need to have a proper knowledge on the physical, chemical and molecular aspects of biomolecules for understanding physiological phenomena of crops and their improvement.

 

Course Learning Outcomes (CLO):

1. Describe scope and importance of biochemistry and molecular biology in agriculture.
2. Explain biological functions, physical and chemical properties of biomolecules.
3. Compare, contrast and interaction among various biomolecules in living system.
4. Construct the structures of various biomolecules
5. Construct recombinant DNA and clones for development of new crop variety.

 

Mapping CLO with PLO

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1	××	××
CLO2	××	××
CLO3	××	××	××
CLO4	××	××	××
CLO5	××	××	××

 

Summary of Course Content

Sl No.	Content	Aligned CLO	No. of Lectures
1	Scope and importance of Biochemistry and Molecular Biology.	CLO1	1
2	Carbohydrates: Classification, function and properties. Chemistry of monosaccharides, disaccharides and polysaccharides, cell wall polysaccharides.	CLO2, CLO3 & CLO4	8
3	Proteins: Properties and classification of proteins and amino acids, reactions of amino acids and peptides, biologically important peptides, organizational level and denaturation of protein.	CLO2, CLO3 & CLO4	6
4	Lipids: Classification and biological functions, Fatty acids: classification and chemical reactions, chemical evaluation and rancidity of fats, Lipids as membrane constituents.	CLO2, CLO3 & CLO4	5
5	Enzyme: Classification and chemical nature, enzyme kinetics, mode of action and inhibition, Factors affecting enzyme activity.	CLO2, CLO3 & CLO4	4
6	Basic molecular biology: Composition, structural features, physio-chemical properties of Nucleic acid, DNA as genetic materials, Central dogma, Genes, Genomes, Genetic code, Basics of Omics technology, Concept of rDNA technology, Molecular basis of stress tolerance in plants.	CLO4 & CLO 5	5
7	Plant Hormones: Classification and biochemical functions. Hormone signaling and mechanism.	CLO2	1
Total No. of Lectures			30

Teaching Strategy

• Lecture
• Group discussion
• Exercise
• Assignment
• Video clip

 Assessment Strategy

• Multiple Choice Question
• Short question
• Essay type question
• Assignment
• Gap filling

Books Recommended: 

1. Lehninger Principles of Biochemistry by David L. Nelson, 2017. 7^th W. H. freeman and company. New York.
2. Textbook of Biochemistry by Thomas M. Devlin, 2002. 5^th John Wiley and Sons, Inc. Printed in USA
3. Biochemistry by Donald Voet, 1995. 2^nd edition, John Wiley and Sons, New York
4. Outlines of Biochemistry by Eric E. Conn and Paul K. Stumpf, 1987. 5^th John Wiley and Sons, New York
5. Biochemistry by Albert L Lehninger, 1982. 2^nd edition Kalyani Publishers. Ludhiana, New Delhi.
6. Harper’s Biochemistry by Robert K Murray, 2002. 25^th McGraw Hill. Printed in Singapore.
7. Biochemistry by Lubert Stryer, 1995. 4^th W. H. freeman and company. New York.
8. Biochemistry by Mary K Campbell, 1999. 3^rd J/B Woolsey Associates. Printed in USA.

 ---------------------------------------------------------------------------------------------------------------------------------------------

Course Code: BMB 1202

Course Title: Biochemistry and Molecular Biology

Credit: 1 (One)

Contact Hours: 2 Hours per week

Level: 1 Semester: 2

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale:

This course will develop the practical skill on various biochemical methods so that the students can use their acquired lab skill for various biochemical researches.

 

Course Learning Outcomes (CLO):

1. Prepare various types of solutions
2. Identify biomolecules from biological samples
3. Measure the activity of enzymes
4. Analyze vitamin, moisture, fat, crude fibre and ash from biological samples
5. Extract and estimate starch, DNA and RNA

Mapping CLO with PLO

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1		××		××
CLO2		××		××
CLO3		××		××			××
CLO4		××		××			××
CLO5		××		××			××

 

Summary of Course Content

Sl No.	Content	Aligned CLO	No. of Lectures
1	Preparation of solutions and their standardization	CLO1	2
2	Preparation of buffer solutions and determination of pH	CLO1	1
3	Qualitative analysis of carbohydrates and proteins	CLO2	4
4	Extraction of starch from natural sources and assay of enzyme action	CLO3 & CLO5	1
5	Quantitative analysis of enzymatic and non-enzymatic antioxidants	CLO4	2
6	Extraction and estimation of DNA and RNA	CLO5	1
7	Proximate analysis: Moisture, fat, crude fibre and ash	CLO4	1
8	Biochemical and molecular laboratory visit	CLO5
Total No. of Lectures			12

 

Teaching Strategy

• Lecture
• Group discussion
• Demonstration
• Assignment
• Practice

 Assessment Strategy

• Multiple Choice Question
• Short question
• Assignment
• Gap filling
• Viva-Voce

Books Recommended: 

1. An introduction to practical Biochemistry. Davit T. Plummer. Tata McGraw-Hill Publishing Company Limited, New Delhi, 1995.
2. Biochemistry Laboratory Manual. F. M. Strong. WM.C. Brown Company Publishers, USA, 1965.
3. Biochemistry Laboratory Techniques. Sterling Chaykin. Wiley Eastern Private Limited, New Delhi, 1970.
4. Biochemical Calculations. How to Solve Mathematical Problem in General Biochemistry. Irwin H. Segel. John Wiley and Sons, Inc. New York, 1968.
5. Experimental Biochemistry. A Laboratory Manual. Gerald Litwack. John Liley and Sons. Inc, New York, 1960.
6. Official Methods of Analysis. Association of Official Analytical Chemists (AOAC), Washington D.C., 1990.

-----------------------------------------------------------------------------------------------------------------------------------------------

Course Code: BMB 2101

Course Title: Metabolism and Human Nutrition

Credit: 3 (Three)

Contact Hours: 3 Hours per week

Level: 2 Semester: 1

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale:

This course covers the mechanism of metabolic processes and their impact on human nutrition under normal and adverse condition.

 

Course Learning Outcomes (CLO):

1. Describe digestion and absorption of nutrient molecules
2. Explain various metabolic processes (pathways / cycles) with their relationship and energetics.
3. Interpret the biochemical function and deficiency symptoms of vitamins and minerals
4. Evaluate the food protein quality, BMR, BMI, RQ and SDA according to age, sex and weight.
5. Construct the relationship between nutrition-sensitive agriculture and national nutritional policy

 

Mapping CLO with PLO 

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1	××
CLO2	××			××
CLO3	××		××
CLO4	××		××						××
CLO5	××							××

 

Summary of Course Content

Sl No.	Content	Aligned CLO	No. of Lectures
1	Bioenergetics: Thermodynamics, Free energy, entropy and enthalpy. Exergonic and endergonic reaction, ADP-ATP cycle.		3
2	Digestion, absorption and basic metabolic concept of biomolecules.		2
3	Carbohydrate Metabolism: Glycolysis and alcoholic fermentation. Krebs cycle. Electron transport chain.  Shuttle systems. Pentose phosphate pathway. Gluconeogenesis, Biosynthesis of sucrose and starch.		8
4	Protein metabolism: Catabolic and anabolic processes of amino acid.  Assimilation of ammonia in plants. Nitrogen cycle. Urea cycle.		4
5	Fat Metabolism: Lipolysis, oxidation of fatty acids, ketone bodies and their fates, glyoxylate cycle, fatty acid biosynthesis.		6
6	Nucleic acid metabolism: Replication, transcription and translation: action, modification and regulation.		4
7	Food protein quality evaluation: Biological value, PER, NPU, PDCAAS.		2
8	Vitamins and minerals: Occurrence, biochemical functions and deficiency symptoms, RDA		3
9	Nutrient contents and availability of basic food groups: Cereals, legumes, oil seeds, fruits, vegetables etc., role of nutrition in growth and health, antinutritional factors, dietary fibre.		3
10	Energy: Requirement according to age, sex and weight; BMR, BMI, RQ, balanced diet, GI food stuffs, role of diet in the development of lifestyle diseases (CVD, DM and cancer). Designing of diet.		4
11	Nutrition and agriculture: National nutritional policy. Crop diversification in relation to human nutrition, Nutritional enhancement of plant food		1
Total No. of Lectures			40

 Teaching Strategy

• Lecture
• Group discussion
• Exercise
• Assignment
• Video clip

 Assessment Strategy

• Multiple Choice Question
• Short question
• Essay type question
• Assignment
• Gap filling

 Books Recommended:

1. Applied Human Nutrition by F. Ann Walker, 1990. Ellis Horwood Limited, West Sussex, England,
2. Lehninger Principles of Biochemistry by David L. Nelson, 2008. 5^th W. H. freeman and company. New York.
3. Textbook of Biochemistry by Thomas M. Devlin, 2002. 5^th John Wiley and Sons, Inc. Printed in USA.
4. Biochemistry by Donald Voet, 1995. 2^nd edition, John Wiley and Sons, New York.
5. Outlines of Biochemistry by Eric E. Conn and Paul K. Stumpf, 1987. 5^th John Wiley and Sons, New York.
6. Biochemistry by Albert L Lehninger, 1982. 2^nd edition Kalyani Publishers. Ludhiana, New Delhi.
7. Harper’s Biochemistry by Robert K Murray, 2002. 25^th McGraw Hill. Printed in Singapore.
8. Biochemistry by Lubert Stryer, 1995. 4^th W. H. freeman and company. New York.
9. Hand Book of Food and Nutrition by M. Swasminathan Ganesh and Company, 1977. Madras, India.
10. Nutrition in Health and Disease by S. Helen Mitchell, 1976. J.B. Lippincott Company, Philadelphia.
11. TextBook of Biochemistry by Edward S. West, Wilber R. Todd, Haward S. Mason and John T. Van Bruggan. 4^th Edition, 1966. The MaCmillan Company. Collier-MaCmillan Ltd. London.

-------------------------------------------------------------------------------------------------------------------------------------------------

Course Code: BMB 2102

Course Title: Metabolism and Human Nutrition

Credit: 1 (One)

Contact Hours: 2 Hours per week

Level: 2 Semester: 1

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale:

This course will develop the practical skill on various biochemical methods and techniques to facilitate acquiring skills for carrying out various biochemical analyses.

Course Learning Outcomes (CLO):

1. Prepare various media for bacteria culture.
2. Characterize fats and oils from biological samples.
3. Quantify proteins and pigments from plant samples.
4. Determine reducing sugar, amino acids, IpH.
5. Perform DNA amplification.

 

Mapping CLO with PLO 

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1	××	××
CLO2	××	××		××
CLO3	××	××		××
CLO4	××	××
CLO5	××	××					××

 Summary of Course Content

Sl No.	Content	Aligned CLO	No. of Lectures
1	Determination of isoelectric pH of protein	CLO4	2
2	Quantification of reducing sugar	CLO4	2
3	Quality evaluation of plant oils: saponification value, iodine value and acid value	CLO2	2
4	Quantification of protein: Biuret and Kjeldahl method	CLO3	2
5	Extraction and estimation of plant pigments	CLO3	2
6	Preparation of LB media and culture of E. coli	CLO1	1
7	Separation of amino acids and sugars by chromatography	CLO4	1
8	DNA amplification and its evaluation: PCR, gel electrophoresis	CLO5	1
9	Biochemistry and Molecular Biology laboratory visit	CLO5
Total No. of Lectures			13

 

Teaching Strategy

• Lecture
• Group discussion
• Demonstration
• Assignment
• Practice

 Assessment Strategy

• Multiple Choice Question
• Short question
• Assignment
• Gap filling
• Viva-Voce

 Books Recommended: 

1. An introduction to practical Biochemistry. Davit T. Plummer. Tata McGraw-Hill Publishing
2. Company Limited, New Delhi, 1995.
3. Biochemical Calculations. How to Solve Mathematical Problem in General Biochemistry. Irwin H. John Wiley and Sons, Inc. New York, 1968.
4. Biochemistry Laboratory Manual. F. M. Strong. WM.C. Brown Company Publishers, USA, 1965.
5. Biochemistry Laboratory Technioques. Sterling Chaykin. Wiley Eastern Private Limited, New Delhi, 1970.
6. Experimental Biochemistry. A Laboratory Manual. Gerald Litwack. John Liley and Sons. Inc, New York, 1960.
7. Official Methods of Analysis. Association of Official Analytical Chemists (AOAC), Washington D.C., 1990.

 -----------------------------------------------------------------------------------------------------------------------------------------

Course Code: BMB 3201

Course Title: Applied Plant Biochemistry (Elective course)

Credit: 2 (Two)

Contact Hours: 2 Hours per week

Level: 3 Semester: 2

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale:

This course covers the advanced biochemical processes involved in plant growth and development and approaches for solving agricultural problems.

 

Course Learning Outcomes (CLO):

1. Explain cellular compartmentation in metabolic regulation of biochemical processes in plants
2. Describe xenobiotics and secondary metabolites in plants
3. Interpret different stress tolerance mechanism in plants
4. Analyze agricultural problems with probable solutions

 

Mapping CLO with PLO 

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1	××	××
CLO2	××	××	××		××
CLO3	××	××
CLO4	××				××		××

 

Summary of Course Content

Sl No.	Content	Aligned CLO	No. of Lectures
1	Molecular basis and functions of cell organelles.	CLO1	2
2	Compartmentation and regulation of metabolic pathways.	CLO1	3
3	Biochemical and biophysical aspects of photosynthesis.	CLO1	3
4	Metabolic regulation in C3, C4, and CAM Plants.	CLO1	3
5	Glyoxylate cycle. Nitrogen and sulfur assimilation and metabolism.	CLO1	3
6	Role of transporters. Xenobiotics metabolism in plants.	CLO2	3
7	Concepts of plant systems biology. Biochemical, physiological and molecular avenues for combating stress tolerance in plants.	CLO3	5
8	Secondary metabolites in plant defense mechanisms. Phytohormones and signal transduction pathway in plants.	CLO2	4
9	Biochemical approaches for solving agricultural problems in Bangladesh.	CLO3 & CLO4	4
10	Field visit for gathering knowledge on on-going biochemical researches at research institutes.	CLO4	-
Total No. of Lectures			30

 

 Teaching Strategy

• Lecture
• Group discussion
• Exercise
• Assignment
• Video clip

 

Assessment Strategy

• Multiple Choice Question
• Short question
• Essay type question
• Assignment
• Gap filling

 

Books Recommended:

1. Lehninger Principles of Biochemistry. David L. Nelson and Michael M. Cox. 2017. 7^th W. H. freeman and company. New York.
2. Biochemistry and Molecular Biology of Plants. Bob B. Buchanan, Wilhelm Gruissem and Russell L. Jones. 2015. 2^nd edition. Willey Blackwell.
3. Plant Biochemistry. Hans-Walter Heldt and Birgit Piechulla. 2011. 4^th edition. Academic press.
4. Harper's Illustrated Biochemistry. Victor W. Rodwell, David Bender, Kathleen M. Botham, Peter J. Kennelly, P. Anthony Weil. 2018. 31^st edition.

 --------------------------------------------------------------------------------------------------------------------------------------------------------------

Course Code: BMB 4101

Course Title: Advanced Molecular Biochemistry and Biotechnology (Elective course)

Credit: 2 (Two)

Contact Hours: 2 Hours per week

Level: 4 Semester: 1  

Course offering Department:  Department of Biochemistry and Molecular Biology

 

Rationale

The agricultural graduates should be equipped with knowledge of crop improvement and this course offers basic concepts of constructing and sequencing of genes for synthesizing new enzymes and proteins for the development of new varieties.

 

Course Learning outcomes

1. Describe the related terminologies, processes of molecular biology
2. Solve enzyme kinetics related mathematical problems
3. Compare and contrast among various genetic materials
4. Construct rDNA and other related materials of rDNA technology
5. Apply the knowledge of rDNA technology

 

Mapping CLO with PLO 

CLO/PLO	PLO1	PLO2	PLO3	PLO4	PLO5	PLO6	PLO7	PLO8	PLO9	PLO10
CLO1	××	××		××
CLO2	××	××	××
CLO3	××		××				××
CLO4	××	××		××			××
CLO5	××	××			××		××

 Summary of Course Content

Sl. No.	Content	Aligned CLO	No. of Lectures
1	Nucleic acid and genetic material, genetic code.	CLO1	4
2	Central dogma of molecular biology: replication, transcription and translation with modification and regulation.	CLO1	8
3	Biochemical techniques: chromatography, electrophoresis, polymerase chain reaction, centrifugation, blotting.	CLO1	7
4	Enzyme isolation, purification, mechanism of action, kinetics, regulation and immobilization. Determination of Km and Vmax. Allosteric enzymes.	CLO2 & CLO3	6
5	Molecular mechanism of antibiotics resistance. Recombinant DNA and concept of biotechnology.	CLO4	5
6	Field Trip to different research organizations and industries.	CLO4 & CLO5
Total No. of Lectures			30

 

Teaching strategy

• Lecture
• Question & answering
• Tutorial
• Self-study /e-learning

Assessment strategy

• MCQ
• Short question
• Essay type question
• Assignment

Books Recommended:

1. Molecular Biology of Gene. 3^rd D. Watson. W. A. Benjamin, Inc. 1987.
2. Biochemistry and Molecular Biology, William H. Elliot and Daphne C. Elliot. 0xford University Press, 1997.
3. Biochemistry, Albert L. Lehninger, 2^nd Kalyani Publishers. Ludhiana, New Delhi, 1982.
4. Molecular Cloning: DNA Analysis. T.A. Brown. 4^th Edition, 2002.
5. Harper’s Biochemistry. R.K. Murray, 25^th McGraw Hill, Printed in Singapore, 2002.
6. L. Stryer, 4^th edition. W.H. Freeman & Company, New York, 1995.
7. Outlines of Biochemistry. E.E. Conn and P.K. Stumpf, John Wiley and Sons, Inc. New York, 1987.
8. Principles of Biochemistry. D.L. Nelson, Lehninger, 5^th W.H. Freeman & Company, New York, 2005.
9. Practical Biochemistry (Principles and Technique) 4^th K. Wilson and John Walker, Cambridge University Press, U.K.
10. An Introduction to Practical Biochemistry. Davit T. Plummer. Tata McGraw-Hill Publishing Company Limited, New Delhi, 1995.
11. HPLC of macromolecules a practical approach. R.W.A. Oliver. IRL press. Walton Street Oxford, 1991.
12. Immobilized cells and enzymes a practical approach. J. Woodward. IRL press. Oxford, Washington DC, 1985.